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KMID : 0382619950150020469
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Hanyang Journal of Medicine
1995 Volume.15 No. 2 p.469 ~ p.478
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An immunohistochemical study of desmoglein I and desmoplakin 1/2 expression after blocking with autoimmune pemphigus antisera
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Ro Young-Suck
Lee Chang-Woo
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Abstract
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Recent studies have shown that pemphigus foliaceus(Pf) antibodies bind to a calciumsensitive epitope on a 160-kd desmosomal glycoprotein, desmoglein 1 (Dsg1), and that pemphigus vulgaris(PV) sera react with a 130-kd polypeptide, desmoglein 3, which is thought to be a member of the desmoglein family.
We investigated the immunohistochemical expression of desmosomal components, Dsg1 and desmoplakin 1/2(Dp1/2), in frozen sections of normal human skin after blocking with Pf sera and with Pv sera.
The results were as follows;
1. There was a complete loss of characteristic membraneous intercellular staining pattern in the sections which were reacted with anti-Dsg1 for 30 minutes after blocking with the undiluted Pf sera. But this staining pattern began to reappear as Pf serum was diluted and incubation time with anti-Dsg1 was increased.
2. There was a preservation of characteristic intercellular staining pattern in any sections which were reacted with anti-Dsg1 after blocking with Pv sera.
3. There was also a preservation of characteristic granular intercellular staining pattern in the section which were reacted with anti-Dp1/2 after blocking with Pf sera and with Pv sera.
These findings suggest that the only Pf sera can block Dsg1 binding with its antibody and the intensity of the reaction seems to be influenced by many factors, such as incubation time, titer and dilution of serum because immunohistochemistry is an enzymatic process.
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